Overview:
Thermo Fisher Scientific’s Invitrogen TrueTag Donor DNA Kits* provide a straightforward toolset to simplify and accelerate cell line engineering with CRISPR-Cas9 or TALEN technology. With the kits, researchers can enrich tagged cells to obtain up to 100% of edited cells. This enables them to skip the time-consuming clonal isolation step and save up to four months on their genome editing experiments compared to traditional gene tagging workflows. Adding to time savings, these off-the-shelf kits provide templates and clean-up columns to generate donor DNA with a one-step PCR workflow — no cloning required.
The expanded portfolio of TrueTag Donor DNA Kits supports four types of locus-specific knock-in templates:
- Fluorescent tagging kits: Add a fluorescent tag to visualize and study localization of an expressed protein.
- Epitope tagging kits: Add an epitope tag to a protein for isolation and localization studies when an antibody is not available.
- Stem cell fluorescent reporter tagging kits: Tag a protein to observe activation behaviors upon stem cell differentiation.
- Knockout enrichment kits: Knock out a gene of interest via insertion of stop codons, plus markers to enrich and visualize edited cells.
Thermo Fisher offers TrueTag Donor DNA Kits for fluorescent and epitope tags including GFP, RFP, BFP, Myc, HA, 6xHis, YFP and DDK
Key Applications:
Genome editing experiments including creating cell lines for disease model studies and reporter assays; tagging genes for localization and isolation studies; enriching cells with functional gene knockout
Featurs/Benefits:
- Reduced protocol time: One-step PCR workflow eliminates the need for cloning, providing a transfection-ready donor DNA template in hours instead of days.
- Maximized editing efficiency: Reduced time and effort to reach up to 100% of edited cells.
- Minimized screening time: Users can enrich edited cells using puromycin or blasticidin.
- User friendly: Comes with easy-to-follow protocols so even novice users can perform successful genome editing experiments.
- Integrated software: Complementary Invitrogen TrueDesign Genome Editing software for easily designing and ordering gene-specific components for each target gene
Website: thermofisher.com/truetag
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. is the world leader in serving science, with annual revenue of approximately $40 billion. Our Mission is to enable our customers to make the world healthier, cleaner and safer. Whether our customers are accelerating life sciences research, solving complex analytical challenges, increasing productivity in their laboratories, improving patient health through diagnostics or the development and manufacture of life-changing therapies, we are here to support them. Our global team delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industry-leading brands, including Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific, Unity Lab Services, Patheon and PPD. For more information, please visit www.thermofisher.com.
